Fig. 5. LC3II/LC3I ratio and Beclin protein expressions in Tacrolimus-, Sirolimus- and Everolimus-treated HepG2 (A and B, respectively) and Huh7 (C and D, respectively) cells. Treatments were administered at different concentrations (0, 10 nM, 10 µM, and 100 µM). The protein expression of LC3II, LC3I and Beclin (6 h) was determined by Western‐blot analysis described in Material and Methods. Results are expressed as mean ± SEM, and blots are representative of three to five independent experiments. *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001 between control and immunosuppressant‐treated cells. The groups with different letters (a, b, c, d, or e) were significantly different (p ≤ 0.05).